Studies of inositol phospholipid-specific phospholipase C
نویسندگان
چکیده
منابع مشابه
Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C.
Phosphoinositides (PIs) constitute a minor fraction of total cellular lipids in all eukaryotic cells. They fulfill many important functions through interaction with a wide range of cellular proteins. Members of distinct inositol lipid kinase families catalyze the synthesis of these phospholipids from phosphatidylinositol. The hydrolysis of PIs involves phosphatases and isoforms of PI-specific p...
متن کاملGenome Analysis Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C
Phosphoinositides (PIs) constitute a minor fraction of total cellular lipids in all eukaryotic cells. They fulfill many important functions through interaction with a wide range of cellular proteins. Members of distinct inositol lipid kinase families catalyze the synthesis of these phospholipids from phosphatidylinositol. The hydrolysis of PIs involves phosphatases and isoforms of PI-specific p...
متن کاملPartial purification and characterization of a (glycosyl) inositol phospholipid-specific phospholipase C from peanut.
We have isolated a glycosyl inositol phospholipid (GIP) anchor-hydrolyzing activity from peanut seeds by a series of column chromatographic steps. The activity has a pH optimum below 6.0, requires calcium, and is inhibited by sulfhydryl reagents. It cleaves the GIP anchors of solubilized acetylcholinesterase from bovine erythrocytes and variant surface glycoprotein from Trypanosoma brucei. On t...
متن کاملInositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.
Antibodies against an inositol phospholipid-specific phospholipase C purified from bovine brain were used to screen rat brain lambda gt11 expression cDNA libraries. Complete sequences of three cDNA inserts yielded a cumulative sequence of 5106 base pairs. The deduced protein had 1289 amino acids with a calculated molecular weight of 148,431. The determination of an open reading frame was aided ...
متن کاملSTUDIES ON PHOSPHOLIPASE C FROM MELIA AZADIRACHTA SEEDS
The activity of phospholipase C in crude enzymatic preparation of Melia azadiracht seeds (Neem seeds) was studied by the use of lecithin as a substrate in aqueous medium. The enzyme activity was found optimum at pH 2.5 and temperature 35?C. The phospholipase C was found heat labile, being inactivated 88% within 10 minutes at 90°C
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ژورنال
عنوان ژورنال: Science
سال: 1989
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.2541501